Validation of an HPLC/UV-based method for <i>Salicornia herbacea</i>-derived isorhamnetin-3-<i>O</i>-glucoside and quercetin-3-<i>O</i>-glucoside quantification

Salicornia herbacea is a type of salt marsh plant that has been used in traditional medicine to treat several diseases. Isorhamnetin-3-O-glucoside (I3G) and quercetin-3-O-glucoside (Q3G) are major flavonoids S. known exert various pharmacological activities. Therefore, our study sought validate optimize an HPLC/UV-based analytical method for I3G Q3G yield quantification, as well determine its limit detection, linearity, precision, accuracy. Upon testing concentration range 31.5-1.9 μg/mL the results exhibited good linearity (r2 ≥0.9996 r2 ≥0.9999 Q3G, respectively), procedure was deemed precise (relative standard deviation ≤3.19 ≤3.85%, accurate (102.6-105.0 92.9-95.2%, respectively). The showed proposed could be rapid evaluation herbacea.